光纤表面等离子体共振葡萄糖浓度传感器研究

郑万禄; 马遥; 张亚男

doi:10.16383/j.aas.c200264

光纤表面等离子体共振葡萄糖浓度传感器研究

doi: 10.16383/j.aas.c200264

1.
东北大学信息科学与工程学院沈阳 110819

基金项目: 国家自然科学基金(61703080, 6177310)资助项目

详细信息

作者简介:
郑万禄：东北大学信息科学与工程学院硕士研究生. 2016年获得河南城建学院电气与控制工程学院学士学位. 主要研究方向光纤表面等离子共振传感技术及其生物医学方面的应用.Email: 15612916827@163.com

马遥：东北大学信息科学与工程学院硕士研究生. 2019年获得东北大学秦皇岛分校控制工程学院学士学位. 主要研究方向为海洋重金属检测, 光纤SPR传感技术. E-mail: my1834654775@163.com

张亚男：东北大学教授. 2015年获得东北大学信息科学与工程学院博士学位. 主要研究方向为光纤传感技术, 敏感材料. E-mail: zhangyanan@ise.neu.edu.cn

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- 文章访问数: 30
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- 被引次数: 0
出版历程
- 收稿日期: 2020-04-30
- 录用日期: 2020-06-01

Research on Glucose Concentration Sensor Based on Optical Fiber Surface Plasmon Resonance Technology

1.
College of Information Science and Engineering, Northeastern University, Shenyang 110819

Funds: Supported by National Natural Science Foundation of China (61703080, 6177310)

摘要

摘要: 本文提出一种基于表面等离子体共振(surface plasmon resonance, SPR)的光纤传感器实现了葡萄糖浓度的测量. 该传感器探头采用反射式结构, 金膜镀在光纤表面激发SPR, 然后采用共价结合的方式将葡萄糖氧化酶(Glucose Oxidase, GOD)固定在金膜表面. 随着葡萄糖浓度的增加, 由于GOD和葡萄糖的结合使得探头表面折射率增加, 最终引起传感器谐振波长发生红移. 通过监测谐振波长的偏移量, 即可实现葡萄糖浓度的测量. 实验结果表明: 该传感器对折射率变化的灵敏度可达到2108.6 nm/RIU; 在0-0.5 mg/mL的葡萄糖浓度范围内, 谐振波长随葡萄糖浓度的增加而线性移动, 灵敏度为85.4 nm/(mg/mL); 随着葡萄糖浓度继续增加, GOD的结合位点逐渐减少, 导致光谱偏移量逐渐降低并趋于饱和, 在0.5-1.2 mg/mL的葡萄糖浓度范围内呈现非线性关系.
- 光纤传感器 /
- 表面等离子体共振 /
- 葡萄糖氧化酶 /
- 葡萄糖浓度测量
Abstract: A fiber optic sensor based on surface plasmon resonance (SPR) was proposed to measure the glucose concentration. The sensing head adopted reflective structure, and gold film was coated on the fiber surface for exciting SPR. Then, glucose oxidase (GOD) was covalently bonded on the surface of the gold film. When the glucose concentration increased, the resonance wavelength had red shift due to the refractive index increase caused by the combination of the GOD and the glucose. Therefore, the glucose concentration could be obtained by monitoring the shift of the resonance wavelength. Experimental results showed that the sensitivity of the sensor to refractive index variation could be 2108.6 nm/RIU. The resonance wavelength would linearly change with the increase of glucose concentration in the concentration range of 0-0.5 mg/mL, and the sensitivity could be 85.4 nm/(mg/mL). With the further increase of glucose concentration, the binding site of the GOD gradually decrease, resulting in the saturation of resonance wavelength. Therefore, the relationship between the resonance wavelength and the glucose concentration in the range of 0.5-1.2 mg/mL was nonlinear.
- Fiber optic sensor /
- Surface plasmon resonance /
- GOD /
- Glucose concentration measurement

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图 1 葡萄糖浓度检测系统结构图

Fig. 1 Schematic of glucose concentration sensing system

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图 2 葡萄糖氧化酶GOD固定

Fig. 2 Schematic diagram of GOD immobilization

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图 3 反射式光纤SPR传感器折射率测量图谱

Fig. 3 Experimental spectra for refractive index measurement

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图 4 灵敏度拟合曲线

Fig. 4 Sensitivity curve for refractive index

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图 5 0-1.2 mg/mL葡萄糖浓度测量输出图谱

Fig. 5 Experimental spectra for different concentration of glucose (0-1.2 mg/mL)

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图 6 葡萄糖浓度测量灵敏度拟合曲线

Fig. 6 Sensitivity curve for glucose concentration measurement

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